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Extracellular Signalling Modulates Scar/WAVE Complex Activity through Abi Phosphorylation.

Shashi Prakash Singh¹, Peter A Thomason¹, Robert H Insall^1,2

¹CRUK Beatson Institute, Glasgow G61 1BD, UK.

|December 24, 2021

Summary

This summary is machine-generated.

Cell migration relies on the Scar/WAVE complex. In Dictyostelium, Abi subunit phosphorylation by extracellular signals fine-tunes pseudopod dynamics and cell speed after Scar/WAVE activation.

Keywords:

F-actin Scar/WAVE complex migration phosphorylation pseudopodia

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Area of Science:

Cell Biology
Biochemistry
Molecular Biology

Background:

The Scar/WAVE complex is essential for actin-based cell migration, controlling lamellipodia and pseudopodia formation.
Regulation of the Scar/WAVE complex is a key determinant of cell motility.

Purpose of the Study:

To investigate the role of Abi subunit phosphorylation in the Scar/WAVE complex during cell migration.
To determine how extracellular signals modulate Scar/WAVE complex activity and pseudopod dynamics.

Main Methods:

Phosphorylation analysis of the Abi subunit in Dictyostelium cells.
Creation and analysis of unphosphorylatable and phosphomimetic Abi mutants.
Assessment of chemotaxis, pseudopodia formation, and cell speed in mutant Dictyostelium.

Main Results:

Abi, but not Scar, is phosphorylated in response to extracellular signaling, occurring after Scar/WAVE complex activation.
Abi phosphorylation is influenced by environmental cues like cell-substrate adhesion and osmolarity.
While not essential for Scar/WAVE activation, Abi phosphorylation affects pseudopod protrusion duration, altering cell speed. Unphosphorylatable Abi leads to faster migration.

Conclusions:

Extracellular signals modulate cell migration by tuning Scar/WAVE complex behavior post-activation through Abi phosphorylation.
Abi phosphorylation acts as a regulatory mechanism influencing pseudopod dynamics and overall cell motility, rather than initiating complex activation.