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Updated: Oct 9, 2025
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Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
Published on: May 22, 2012
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The CWI Pathway: A Versatile Toolbox to Arrest Cell-Cycle Progression.
Inma Quilis1, Mercè Gomar-Alba1, Juan Carlos Igual1
1Departament de Bioquímica i Biologia Molecular, Institut Universitari de Biotecnologia i Biomedicina (BIOTECMED), Universitat de València, 46100 València, Spain.
Journal of Fungi (Basel, Switzerland)
|December 24, 2021
Summary
The cell-wall integrity (CWI) pathway in yeast acts as a crucial brake, halting cell-cycle progression during environmental stress. This pathway ensures cell survival by coordinating responses to damage and maintaining genetic integrity.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Cell-signaling pathways enable cells to adapt to environmental changes.
- The cell-wall integrity (CWI) pathway in *Saccharomyces cerevisiae* responds to cell wall damage and stress.
- CWI pathway activation is linked to cell-cycle progression and polarized growth.
Purpose of the Study:
- To elucidate the role of the CWI pathway in regulating cell-cycle transitions.
- To investigate how CWI pathway signaling impacts cell-cycle progression in response to cell surface perturbation.
- To explore the connection between the CWI pathway, genotoxic stress, and DNA integrity checkpoints.
Main Methods:
- Analysis of cell-cycle progression under various stress conditions.
- Investigating the regulatory mechanisms of the CWI pathway on cell-cycle transitions.
- Examining the interplay between CWI signaling and DNA integrity checkpoints.
Main Results:
- The CWI pathway delays cell-cycle progression at critical transitions like G1/S and mitosis.
- It regulates the Start transcriptional program and DNA replication initiation.
- The pathway connects to the DNA integrity checkpoint, crucial for cell survival under genotoxic stress.
Conclusions:
- The CWI pathway acts as a master regulator, halting cell-cycle progression during unfavorable conditions.
- It plays a vital role in maintaining cellular homeostasis and survival by integrating stress responses with cell-cycle control.
- This pathway is essential for preventing the propagation of damaged cells.
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