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In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
Published on: September 20, 2021
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Three-dimensional transistor arrays for intra- and inter-cellular recording.
Yue Gu1,2, Chunfeng Wang2, Namheon Kim2
1Materials Science and Engineering Program, University of California San Diego, La Jolla, CA, USA.
Nature Nanotechnology
|December 24, 2021
Summary
Researchers developed a scalable platform using field-effect transistors for accurate electrophysiology recordings. This technology precisely measures cellular electrical signals, advancing the study of cell communication and function.
Area of Science:
- Electrophysiology
- Biophysics
- Bioelectronics
Background:
- Electrophysiology relies on accurate electrical impulse recording, but current technologies face limitations in sensing accuracy and scalability.
- Advancing electrophysiology requires improved methods for recording transmembrane potentials in excitable cells.
Purpose of the Study:
- To introduce a novel, scalable platform for accurate recording of transmembrane potentials in electrogenic cells.
- To overcome the limitations of current electrophysiological recording techniques.
Main Methods:
- Development of a scalable platform utilizing a three-dimensional high-performance field-effect transistor (FET) array.
- Minimally invasive cellular interfacing with FETs for faithful transmembrane potential recordings.
- Validation of the platform against the gold standard patch clamp technique.
Main Results:
- Accurate recording of transmembrane potentials in electrogenic cells demonstrated.
- Measurement of cardiomyocyte intracellular signal conduction velocity at 0.182 m/s, five times the intercellular velocity.
- Successful intracellular recordings in cardiac muscle tissue constructs, revealing signal conduction paths.
Conclusions:
- The developed FET-based platform offers a scalable solution for accurate electrophysiological recordings.
- The platform enables high spatial and temporal resolution measurements of cellular electrical activity.
- This technology has broad implications for understanding cellular physiology, pathology, and cell-cell interactions in various biological systems.
