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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
Strategies for Optimization of the Clustered Regularly Interspaced Short Palindromic Repeat-Based Genome Editing
Yang-Min Wang1,2, Hao-Zheng Wang1,2, Ying-Zhen Jian1,2
1Guang dong Province Key Laboratory of Biotechnology Drug Candidates, Guang dong Pharmaceutical University, Guang zhou, P.R. China.
Abstract:
The clustered regularly interspaced short palindromic repeats (CRISPR) system is inarguably the most valuable gene editing tool ever discovered. Currently, three classes of CRISPR-based genome editing systems have been developed for gene editing, including CRISPR/CRISPR associate system (Cas) nucleases, base editors, and prime editors. Ever-evolving CRISPR technology plays an important role in medicine; however, the biggest obstacle to its use in clinical practice is the induction of off-target effects (OTEs) during targeted editing. Therefore, continuous improvement and optimization of the CRISPR system for reduction of OTEs is a major focus in the field of CRISPR research. This review aims to provide a comprehensive guide for optimization of the CRISPR-based genome editing system.
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