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Using Microinjection of Mammalian Cultured Cells to Study Cell Division
Charles Day1,2, Alyssa Langfald1, Edward H Hinchcliffe3,4
1The Hormel Institute, University of Minnesota, Austin, MN, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2022
Summary
Microinjection delivers macromolecules into single mitotic cells for manipulation. This technique, using simple equipment, allows researchers to study cellular processes via live imaging.
Area of Science:
- Cell Biology
- Molecular Biology
Background:
- Microinjection is a key technique for introducing molecules into cells.
- Manipulating mitotic cells is crucial for understanding cell division.
Purpose of the Study:
- To describe a microinjection setup for cultured mammalian cells.
- To detail the process of microinjecting cells in mitosis.
- To explain analysis using same-cell live and fixed imaging.
Main Methods:
- Utilizing a microinjection system.
- Injecting macromolecules (mRNA, proteins, antibodies) into individual mitotic cells.
- Performing live-cell imaging and subsequent fixed imaging on the same cells.
Main Results:
- Demonstration of a functional microinjection setup.
- Successful introduction of various macromolecules into mitotic cells.
- Effective analysis of cellular responses through correlative imaging.
Conclusions:
- Microinjection is an accessible and powerful method for studying mitotic cell biology.
- The described technique enables precise manipulation and detailed analysis of cellular events.
- This approach facilitates research in cell cycle regulation and drug discovery.
