Preparation of Platelet-Rich Plasma: National IADVL PRP Taskforce Recommendations

Area of Science:

• Dermatology
• Regenerative Medicine
• Biotechnology

Background:

• Platelet-rich plasma (PRP) is increasingly utilized in dermatology for various indications.
• Standardized protocols for PRP preparation are lacking, leading to variability in clinical outcomes.
• The Indian Association of Dermatologists, Venereologists and Leprologists (IADVL) established a task force to develop evidence-based recommendations.

Purpose of the Study:

• To establish a framework for practitioners on the evidence-based preparation of platelet-rich plasma (PRP).
• To provide specific recommendations for PRP preparation in dermatological applications.
• To ensure consistency and efficacy in PRP-based dermatological treatments.

Main Methods:

• A comprehensive literature search was conducted across multiple databases for English-language articles on PRP preparation.
• The GRADE framework was used to evaluate the grade of evidence and strength of recommendations.
• A draft of recommendations was developed, scrutinized, and critically evaluated by the IADVL Academy of Dermatology.

Main Results:

• A total of 45 articles, including meta-analyses, studies, and reviews, were evaluated.
• The double-spin manual method is recommended for PRP preparation.
• Recommended centrifuge parameters: 100-300 g for 5-10 min (1st spin), 400-700 g for 10-17 min (2nd spin).
• Optimal platelet concentration: 1-1.5 million platelets/μL.
• PRP activation is not required for injection into soft tissues.

Conclusions:

• These recommendations provide a standardized, evidence-based approach to PRP preparation in dermatology.
• The defined protocol aims to optimize therapeutic outcomes in dermatological conditions treated with PRP.
• Practitioners can confidently implement these guidelines for consistent and effective PRP application.