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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Updated: Oct 8, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
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Liquid-phase scanning electron microscopy for single membrane protein imaging.

Li Wang1, Changshuo Li1, Jintao Li2

  • 1Beijing Key Laboratory of Microstructure and Property of Solids, Institute of Microstructure and Property of Advanced Materials, Faculty of Materials and Manufacturing, Beijing University of Technology, Beijing, 100124, China.

Biochemical and Biophysical Research Communications
|January 3, 2022
PubMed
Summary
This summary is machine-generated.

We developed novel liquid imaging techniques for scanning electron microscopy to observe biological cells in their native liquid state. These methods enable high-resolution visualization of membrane proteins and cellular structures at the molecular level.

Keywords:
CathodoluminescenceLiquid-phase scanning electron microscopyMembrane proteins

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Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • Observing biological samples in their native liquid state at high resolution is crucial for understanding cellular function.
  • Existing electron microscopy techniques often require sample dehydration, altering their native structure.
  • There is a need for advanced imaging methods that preserve cellular integrity in solution.

Purpose of the Study:

  • To develop and validate novel liquid imaging approaches for scanning electron microscopy (SEM) of biological cells.
  • To achieve high-resolution visualization of cellular structures and membrane proteins in their native, hydrated state.
  • To demonstrate the utility of these techniques for studying molecular localization and distribution.

Main Methods:

  • Development of liquid-cell apparatus for scanning transmission electron imaging (LC-STEM).
  • Implementation of correlative cathodoluminescence and electron microscopy (CCLEM) for enhanced imaging.
  • Application of these techniques to observe native biological cells in solution.

Main Results:

  • LC-STEM achieved approximately 2 nm resolution, providing excellent contrast for membrane protein analysis.
  • CCLEM improved the resolution of fluorescence imaging to 10 nm.
  • Demonstrated precise recognition of membrane protein localization, distribution, and configuration on native cells in solution.

Conclusions:

  • Liquid SEM technologies offer unique capabilities for studying cellular structure and function in near-native states.
  • These advanced imaging methods allow for monomolecular-level analysis of cells and membrane proteins.
  • The developed techniques hold significant potential for broad applications in cell biology and related fields.