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Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus
Published on: April 19, 2024
Polydopamine-coated magnetic Spirulina nanocomposite for efficient magnetic dispersive solid-phase extraction of
Rouhollah Karami-Osboo1, Fatemeh Ahmadpoor2, Mahmoud Nasrollahzadeh2
1Mycotoxins Research Laboratory, Agricultural Research Education and Extension Organization (AREEO), Iranian Research Institute of Plant Protection, Iran.
Abstract:
An efficient adsorbent was synthesized and used in magnetic dispersive solid phase extraction (MDSPE) of aflatoxins B1, B2, G1, and G2 at trace levels in pistachio prior to analysis by HPLC equipped with a fluorescence detector. Spirulina (Sp) algae was first magnetized, followed by surface modification with dopamine (Dp). The adsorbent was characterized using FT-IR, XRD, FE-SEM, EDX, VSM, and BET analyses. The effects of different analytical parameters on the extraction performance were evaluated. Under optimal conditions, good limits of detection (LODs) and quantifications (LOQs) were achieved in the ranges of 0.02-0.07 and 0.06-0.21 ng g-1, respectively. The RSDs were 5.9, 6.3, 5.6, and 7.3% for AFB1, G1, B2, and G2, respectively. The proposed method was successfully used to determine AFs in pistachio samples and acceptable recoveries in the range of 72-95% were obtained.
