Hybridoma Screening by Antigen Capture: Immunoprecipitation

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Updated: Oct 8, 2025

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Edward A Greenfield

    Cold Spring Harbor Protocols
    |January 5, 2022
    PubMed

    Immunoprecipitation is rarely used for screening hybridoma fusions because the assays are tedious and time-consuming. However, it can be useful when working with complex antigens because the precipitated antigen is normally detected after sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis and thus it is simple to discriminate between true and false positives. Furthermore, the assay provides information regarding the molecular weight of the antigen.

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