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One-Pot Biosynthesis of 3-Aminopropionic Acid from Fumaric Acid Using Recombinant Bacillus megaterium Containing a
Subbi Rami Reddy Tadi1, Ganesh Nehru1, Senthilkumar Sivaprakasam2
1BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
Applied Biochemistry and Biotechnology
|January 8, 2022
Summary
This study developed an eco-friendly biocatalytic process for producing 3-aminopropionic acid (3-APA) from fumaric acid using engineered Bacillus megaterium. The optimized process achieved a high 3-APA titer, demonstrating a sustainable bio-production method.
Area of Science:
- Biotechnology and Industrial Microbiology
- Metabolic Engineering
- Enzyme Engineering
Background:
- 3-Aminopropionic acid (3-APA) is a valuable chemical with diverse industrial applications.
- Current production methods may have environmental drawbacks.
- Developing sustainable bio-production routes is crucial.
Purpose of the Study:
- To establish an eco-friendly whole-cell biocatalytic process for 3-APA production.
- To utilize fumaric acid as a substrate.
- To engineer Bacillus megaterium for enhanced 3-APA biosynthesis.
Main Methods:
- Engineered a dual-enzyme cascade using aspartate ammonia-lyase (AspA) and aspartate-1-decarboxylases (ADC).
- Optimized enzyme expression levels through gene mining, optimization, and duplication.
- Optimized culture cultivation and biocatalysis parameters (temperature, pH, substrate concentration).
Main Results:
- Achieved a maximum 3-APA titer of 11.68 g/L.
- Obtained a yield of 0.78 g/g fumaric acid.
- Identified optimal conditions: 45°C, pH 6.0, and 15 g/L fumaric acid.
Conclusions:
- Successfully established a recombinant Bacillus megaterium whole-cell biocatalysis system for 3-APA production.
- Demonstrated the feasibility of producing 3-APA from fumaric acid sustainably.
- The optimized process offers a promising alternative to conventional chemical synthesis.
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