Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

778
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
778
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

564
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
564
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

591
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
591

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toward Sustainable Clinical Analysis: Benchmarking Plastic Use in LC-MS Sample Preparation - Exemplified by Ketamine Analogues in Whole Blood.

Analytical chemistry·2026
Same author

Development of electromembrane extraction using deep eutectic solvents for 22 representative veterinary drug residues in honey, milk, and eggs.

Analytical and bioanalytical chemistry·2026
Same author

Microfluidic device for electromembrane extraction with a micro-pillar stabilized liquid membrane.

Analytical and bioanalytical chemistry·2026
Same author

96-Well Agarose-Gel Electromembrane Extraction.

Analytical chemistry·2025
Same author

Automated Formation of Supported Liquid Membranes by Molecular Self-Assembly: A Step Forward for Liquid-Phase Microextraction.

Analytical chemistry·2025
Same author

96-Well liquid membrane extraction of weakly acidic compounds.

Analytica chimica acta·2025
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026
Same journal

Stereo-sensitive modelling of gas chromatographic retention indices of mono-cycloalkanes in jet fuel range.

Journal of chromatography. A·2026
Same journal

Approaches to using retention indices with coupled column pressure tuning in gas chromatography.

Journal of chromatography. A·2026
Same journal

MOF-supported surface-imprinted polymer for hazard governance of aristolochic acids in herbal matrices: A safety-control strategy supported by multiscale simulations.

Journal of chromatography. A·2026
See all related articles

Related Experiment Video

Updated: Oct 7, 2025

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids
06:45

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

1.4K

Membrane-based liquid-phase microextraction of basic pharmaceuticals - A study on the optimal extraction window.

Maria Schüller1, Kim Tu Thi Tran1, Elisabeth Leere Øiestad2

  • 1Department of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, Norway.

Journal of Chromatography. A
|January 8, 2022
PubMed
Summary
This summary is machine-generated.

This study defines the optimal extraction window (OEW) for membrane-based liquid-phase microextraction (MP-LPME) using deep eutectic solvents (DES). The OEW is influenced by analyte polarity and interactions, guiding efficient extraction of diverse compounds.

Keywords:
Deep eutectic solventsHollow fiberLiquid-phase microextractionPharmaceuticalsSample preparation

More Related Videos

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.2K
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

35.7K

Related Experiment Videos

Last Updated: Oct 7, 2025

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids
06:45

Author Spotlight: Optimizing Hollow-Fiber Membranes for Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids

Published on: August 9, 2024

1.4K
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.2K
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

35.7K

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Sample Preparation Techniques

Background:

  • Liquid-phase microextraction (LPME) is a widely used sample preparation technique.
  • Optimizing extraction conditions, particularly the optimal extraction window (OEW), is crucial for efficient analyte recovery.
  • Deep eutectic solvents (DES) offer unique properties as supported liquid membranes (SLM) in membrane-based LPME (MB-LPME).

Purpose of the Study:

  • To define the optimal extraction window (OEW) for three-phase membrane-based liquid-phase microextraction (MP-LPME).
  • To investigate the influence of analyte polarity (log P) and interactions (ionic, hydrogen bond, π-π) on the OEW.
  • To evaluate the role of deep eutectic solvents (DES) and ionic carriers in modulating the OEW for diverse analytes.

Main Methods:

  • Utilized three-phase membrane-based liquid-phase microextraction (MP-LPME) with deep eutectic solvents (DES) as supported liquid membranes (SLM).
  • Investigated eleven basic model analytes with a log P range of -0.4 to 5.0.
  • Employed a 96-well format extraction from phosphate buffer into HCl acceptor solution, with and without the ionic carrier di(2-ethylhexyl) phosphate (DEHP).

Main Results:

  • With pure DES, the OEW was observed for analytes with log P between 2 and 5, favoring non-polar compounds with low SLM aromaticity, achieving up to 98% recovery.
  • Addition of DEHP to SLMs shifted the OEW to analytes with log P between -0.5 and 2, enhancing polar analyte extraction up to 80% with moderate aromaticity.
  • Ionic carriers were inefficient for non-polar analytes due to excessive trapping in the SLM.

Conclusions:

  • Membrane-based liquid-phase microextraction (MP-LPME) demonstrates optimal performance within a narrow log P window of approximately 2-3 units.
  • The OEW is significantly influenced by the presence of ionic carriers and the aromaticity of the supported liquid membrane (SLM).
  • Strategic selection of SLM composition, including ionic carriers and aromaticity, allows for targeted optimization of MP-LPME for specific analyte polarity ranges.