Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Studies on the RAST method using tray added disc].

A Yoshimoto1, A Kawamura, M Chinota

  • 1Kotobiken Medical Laboratory, Tokyo, Japan.

Radioisotopes
|October 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proton Beam Therapy for Intracranial Germ Cell Tumors in Pediatric, Adolescent, and Young Adult Patients: A Single-Center Experience.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Corrigendum to 'Combined intake of astaxanthin, β-carotene, and resveratrol elevates protein synthesis during muscle hypertrophy in mice' Nutrition 69: 110561 (2020) 10.1016/j.nut.2019.110561 1-6.

Nutrition (Burbank, Los Angeles County, Calif.)·2022
Same author

First Report of Pythium aphanidermatum on Basella rubra in Japan.

Plant disease·2019
Same author

Non-competitive cyclic peptides for targeting enzyme-substrate complexes.

Chemical science·2018
Same author

Cluster Analysis by Measurement of Peroxidase and Esterase from Citrus Flavedo.

Bioscience, biotechnology, and biochemistry·2016
Same author

Betti reaction enables efficient synthesis of 8-hydroxyquinoline inhibitors of 2-oxoglutarate oxygenases.

Chemical communications (Cambridge, England)·2015

A new tray-based method for the radioallergosorbent test (RAST) offers a rapid, simple, and economical way to detect specific IgE antibodies. This validated approach correlates well with existing methods, aiding in allergy diagnosis and monitoring seasonal pollen variations.

Area of Science:

  • Immunology
  • Allergy Diagnostics

Context:

  • The radioallergosorbent test (RAST) is a standard method for detecting specific IgE antibodies.
  • Current RAST methods, often using tube-added discs, are widely employed in routine assays.
  • Optimization of RAST procedures is crucial for improving efficiency and cost-effectiveness.

Purpose:

  • To develop and validate a novel RAST method utilizing a tray-added disc system.
  • To evaluate the performance characteristics, including correlation, rapidity, simplicity, and economics, of the new tray-based RAST method.
  • To assess the intra-assay and inter-assay variability of the developed method.

Summary:

  • A new RAST method using a tray-added disc system was developed and showed good correlation with the original tube-based method.

Related Experiment Videos

  • The tray method is characterized by its rapidity, simplicity, and economic advantages.
  • Intra-assay coefficients of variation ranged from 9.40-9.68%, and inter-assay coefficients of variation ranged from 10.39-12.15%.

    • Impact:

    • The tray-based RAST method presents a highly useful alternative for routine allergy diagnostics.
    • This method facilitates the estimation of seasonal variations in positive allergens.
    • Monitoring seasonal RAST variations can provide valuable insights into regional pollen allergy burdens and environmental factors.