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Human Tonsil-Derived Mesenchymal Stem Cells-Loaded Hydroxyapatite-Chitosan Patch for Mastoid Obliteration
Sung-Won Choi1, Jieun Kang1, Caifeng Wang2
1Department of Otorhinolaryngology and Pusan National University School of Medicine, Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Republic of Korea.
ACS Applied Bio Materials
|January 12, 2022
Summary
Human tonsil-derived mesenchymal stem cells combined with a hydroxyapatite-chitosan patch significantly enhanced bone formation in temporal bone defects. This tissue engineering approach shows promise for reconstructing surgical bone defects.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Otolaryngology
Background:
- Canal wall down (CWD) mastoidectomy for chronic ear diseases creates bone defects.
- Reconstruction of these temporal bone defects remains a clinical challenge.
Purpose of the Study:
- To evaluate the efficacy of human tonsil-derived mesenchymal stem cells (hTMSCs) combined with a hydroxyapatite (HAp)-chitosan patch for promoting osteogenesis.
- To assess the potential of this combination therapy in reconstructing postoperative temporal bone defects using an animal model.
Main Methods:
- An animal model was used to compare the effects of hTMSCs and a HAp-chitosan patch, alone and in combination, on temporal bone defect repair.
- Histopathological observation and computed tomography (CT) imaging were employed to assess bone formation and obliteration ratios at 12 weeks post-surgery.
Main Results:
- Both the Patch group and the hTMSCs + Patch group demonstrated significantly improved obliteration ratios and bone formation compared to control groups.
- The hTMSCs + Patch group exhibited enhanced osteogenesis, with bone formation observed in both peripheral and central regions of the defect cavity.
- The combination therapy significantly outperformed the patch alone.
Conclusions:
- Combining hTMSCs with a HAp-chitosan patch effectively accelerates osteogenesis for the reconstruction of temporal bone defects.
- This hTMSCs + Patch scaffold represents a promising biomaterial for tissue engineering applications in treating bone defects.
