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Updated: Oct 6, 2025
Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
Published on: September 29, 2017
Biocompatible Three-Dimensional Hydrogel Cell Scaffold Fabricated by Sodium Hyaluronate and Chitosan Assisted
Hong Yu1,2, Jie Liu1, Yuan-Yuan Zhao3
1Laboratory of Organic NanoPhotonics and CAS Key Laboratory of Bio-Inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, No. 29, Zhongguancun East Road, Beijing 100190, P. R. China.
Abstract:
The biocompatibility of the three-dimensional (3D) hydrogel cell scaffolds that sodium hyaluronate (SH) and carboxymethyl chitosan (Chitosan) has been investigated. The minimum processing threshold of 2.94 mW and the feature line width of 80 nm have been obtained by the two-photon polymerization (TPP) technology using the as-prepared biocompatible photoresist. Through tuning the contents of the cross-linker in the hydrogel, the mechanical property of 3D hydrogel scaffold can be optimized to ensure the stability and affinity of scaffold. Furthermore, we have fabricated the SH and Chitosan assisted 3D hydrogel cell scaffolds and investigated the biocompatibility. This study provides a facile method to develop 3D biocompatible hydrogel cell scaffold, which would open up new avenues for the potential application in tissue engineering.
