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[Reference values of serum creatinine measured automatically by the Jaffé fixed-time reaction]
R Grisler1, R Scaricabarozzi, L Xaiz
1Laboratorio di ricerche cliniche, anatomia ed istologia patologica, Istituti Clinici di Perfezionamento, Milano.
Quaderni Sclavo Di Diagnostica Clinica E Di Laboratorio
|September 1, 1987
Summary
This study validates the Jaffé reaction for automated creatinine measurement in serum samples. The automated method demonstrated high precision and accuracy, aligning well with enzymatic procedures for reliable clinical use.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Analytical Chemistry
Context:
- Serum creatinine levels are crucial biomarkers for kidney function assessment.
- Accurate and reliable measurement of creatinine is essential for clinical diagnosis and patient management.
- Traditional methods for creatinine analysis can be time-consuming and labor-intensive.
Purpose:
- To evaluate the performance of an automated fixed-time Jaffé reaction for serum creatinine determination.
- To assess the imprecision and inaccuracy of the automated method using within-run and between-day analyses.
- To compare the results obtained from the automated Jaffé reaction with those from a standard enzymatic colorimetric method.
Summary:
- 1077 serum samples with creatinine levels ≤1.4 mg/dl were analyzed using the 430 Selective Analyzer Sclavo with a fixed-time Jaffé reaction.
- The automated method exhibited very low variation (<3%) and bias coefficients, indicating high precision and accuracy.
- Statistical analysis showed a mean creatinine value of 0.66 mg/dl (DS=0.20 mg/dl), consistent with the enzymatic reference method.
Impact:
- The automated Jaffé reaction offers a reliable and efficient alternative for routine serum creatinine testing.
- This method can improve laboratory workflow and reduce turnaround times for critical diagnostic information.
- Validated automated creatinine assays contribute to more accurate kidney function assessment and patient care.