Related Experiment Video
Updated: Oct 5, 2025
Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection
Published on: July 28, 2022
Negeviruses isolated from mosquitoes in the Brazilian Amazon
Ana Cláudia da Silva Ribeiro1, Lívia Caricio Martins1, Sandro Patroca da Silva1
1Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil.
Background:
There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon Negevirus, a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells.
Methods:
Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed.
Results:
Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon Negevirus: The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus.
Conclusions:
We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil: Cordoba virus and the Negev virus and, a new virus for science: the Feitosa virus.
