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Updated: Oct 5, 2025
Use of Human Perivascular Stem Cells for Bone Regeneration
Published on: May 25, 2012
Healing of bone defects by induced pluripotent stem cell-derived bone marrow mesenchymal stem cells seeded on
Lishen Zhou1, Renfu Quan1, Jun Yang1
1Department of Orthopedic Surgery, Xiaoshan Hospital of Traditional Chinese Medicine, Hangzhou, China.
Background:
Induced pluripotent stem cells (iPSCs) can generate bone marrow mesenchymal stem cells (BMSCs) as seed cells for tissue-engineered bone to repair bone defects. In this study, we investigated the effects of hydroxyapatite-zirconia (HA/ZrO2) composites combined with iPSC-derived BMSCs as a bone substitute on repairing skull defects in rats.
Methods:
Human urinary cells isolated from a healthy donor were reprogramed into iPSCs and then induced into BMSCs. Immunocytochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR) were used to examine the characteristics of the induced MSCs. The iPSC-derived BMSCs were cultured on HA/ZrO2 composites, and cytocompatibility of the composite was analyzed by cell counting kit-8 (CCK-8) assays, RT-PCR, and scanning electron microscopy. Then, HA/ZrO2 combined with iPSC-derived expanded potential stem cells (EpSCs) were transplanted onto skull defects of rats. The effects of this composite on bone repair were evaluated by IHC.
Results:
The results showed that MSCs induced from iPSCs displayed the phenotypes and property of normal BMSCs. After seeding on the HA/ZrO2, iPSC-derived BMSCs had the ability to proliferate and differentiate into osteoblasts. After transplantation, iPSC-derived BMSCs on HA/ZrO2 promoted construction of bone on rat skulls.
Conclusions:
These results indicated that transplantation of a HA/ZrO2 combined with iPS-derived BMSCs is feasible to reconstruct bones and may be a substantial reference for iPSC-based therapy for bone defects.
