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Updated: Oct 5, 2025

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres
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Chitosan-collagen-hydroxyapatite membranes for tissue engineering.

José Becerra1,2, Mariano Rodriguez3, Dayana Leal1

  • 1Instituto de Ciencias Básicas, Universidad Técnica de Manabí, Portoviejo, Ecuador.

Journal of Materials Science. Materials in Medicine
|January 24, 2022
PubMed
Summary

New chitosan/collagen/hydroxyapatite membranes show excellent cell adhesion and low cytotoxicity. These advanced biomaterials hold significant potential for tissue engineering applications, promoting connective tissue regeneration.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Degenerative pathologies necessitate advanced solutions in connective tissue repair.
  • Biocompatible, bioactive, and biodegradable materials are crucial for effective tissue regeneration.
  • Chitosan, collagen, and calcium phosphates are promising candidates for composite biomaterials.

Purpose of the Study:

  • To develop and characterize chitosan/collagen/hydroxyapatite composite membranes for tissue engineering.
  • To evaluate cell adhesion and cytotoxicity of these novel biomaterials in vitro.
  • To investigate the structural and thermal properties of the composite membranes.

Main Methods:

  • Fabrication of chitosan/collagen/hydroxyapatite membranes with varying compositions.
  • In vitro assessment of cell adhesion using MTS assay.
  • Cytotoxicity evaluation via hemolysis assay.
  • Structural and thermal analysis using XRD, FTIR, SEM, and TGA.

Main Results:

  • Composite membranes exhibited good cell adhesion, with optimal results for high hydroxyapatite and collagen content.
  • All tested membranes demonstrated low cytotoxicity.
  • Structural and thermal analyses confirmed the integrity and stability of the composite materials.

Conclusions:

  • Chitosan/collagen/hydroxyapatite composites are promising biomaterials for tissue engineering.
  • These materials support cell adhesion and exhibit low cytotoxicity.
  • Further research is warranted to explore their therapeutic potential in regenerative medicine.