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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
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[Genetic Engineering Systems to Study Human Viral Pathogens from the Coronaviridae Family]
S O Galkin1,2, A N Anisenko1,2,3, O A Shadrina2,3
1Bioengineering and Bioinformatics Department, Moscow State University, Moscow, 119991 Russia.
Molekuliarnaia Biologiia
|January 27, 2022
Summary
This review summarizes genetic engineering systems for studying SARS-CoV-2 and other coronaviruses. It covers viral genome manipulation and inhibitor screening for developing effective COVID-19 treatments.
Area of Science:
- Virology
- Molecular Biology
- Genetic Engineering
Background:
- The COVID-19 pandemic, caused by SARS-CoV-2, highlighted the need for advanced research tools.
- Understanding coronavirus structure and life cycles is crucial for therapeutic development.
- Existing genetic engineering systems require evaluation for studying SARS-CoV-2 and related viruses.
Purpose of the Study:
- To review current genetic engineering systems for studying SARS-CoV-2 and other Coronaviridae.
- To provide an overview of coronavirus structure and life cycle relevant to genetic manipulation.
- To facilitate high-throughput screening of potential viral inhibitors.
Main Methods:
- Literature review of genetic engineering systems for coronaviruses.
- Compilation of data on SARS-CoV-2 and other Coronaviridae studies.
- Synthesis of information on viral structure and replication.
Main Results:
- Summary of various genetic engineering approaches applicable to coronaviruses.
- Overview of key features of coronavirus genome manipulation.
- Identification of systems suitable for inhibitor screening.
Conclusions:
- Effective genetic engineering systems are vital for advancing coronavirus research.
- Further development of these systems can accelerate the discovery of antiviral therapies.
- This review provides a foundation for selecting appropriate tools for coronavirus studies.
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