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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
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A simple and sensitive AuNPs-based colorimetric aptasensor for specific detection of azlocillin
Shuyan Xiao1, Jiping Lu1, Liang Sun1
1School of Materials and Metallurgy, Inner Mongolia University of Science and Technology, Baotou 014010, China; Inner Mongolia Key Laboratory of Advanced Ceramic Materials and Devices, Baotou 014010, China.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|January 30, 2022
Summary
A novel colorimetric biosensor utilizing DNA aptamers and gold nanoparticles (AuNPs) enables sensitive detection of azlocillin. This method offers a simple, visual, and quantitative approach for identifying azlocillin in various samples.
Area of Science:
- Analytical Chemistry
- Nanotechnology
- Biochemistry
Background:
- Azlocillin is a crucial antibiotic requiring reliable detection methods.
- Existing detection techniques may lack sensitivity or simplicity.
- Colorimetric biosensors offer potential for rapid and visual detection.
Purpose of the Study:
- To develop a novel colorimetric biosensor for the specific detection of azlocillin.
- To utilize DNA aptamers and gold nanoparticles (AuNPs) for azlocillin recognition and signal transduction.
- To establish a sensitive, selective, and visually observable detection method.
Main Methods:
- Development of a colorimetric biosensor based on DNA aptamers and unmodified gold nanoparticles (AuNPs).
- Exploitation of azlocillin-induced aggregation of AuNPs leading to a visible color change (red to blue).
- Quantitative analysis using UV-Vis spectroscopy at 522 nm.
Main Results:
- The biosensor demonstrated specific colorimetric detection of azlocillin.
- A linear relationship was observed between absorbance variation and azlocillin concentration (50–500 nM).
- A low detection limit of 11.6 nM was achieved.
- Successful application in detecting azlocillin in milk and tap water samples with high recovery rates (97.64–102.21%) and low RSD (<3.81%).
Conclusions:
- The developed aptasensor provides a sensitive and selective method for azlocillin detection.
- The naked-eye visual detection and UV-Vis quantification offer versatility.
- This biosensor shows promise for practical applications in real-world sample analysis.
