Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

8.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
8.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

15.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
15.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Potentially functional variants of PLCE1 identified by GWASs contribute to gastric adenocarcinoma susceptibility in an eastern Chinese population.

PloS one·2012
Same author

Pennogenin tetraglycoside stimulates secretion-dependent activation of rat platelets: evidence for critical roles of adenosine diphosphate receptor signal pathways.

Thrombosis research·2012
Same author

Polymorphisms in the XPG gene and risk of gastric cancer in Chinese populations.

Human genetics·2012
Same author

[Influences of D-galactosamine and lipopolysaccharide on liver tissue regeneration and repair in mice with partial hepatectomy].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2012
Same author

Large and giant ventral paraclinoid carotid aneurysms: surgical techniques, complications and outcomes.

Clinical neurology and neurosurgery·2012
Same author

Association of mitochondrial DNA variations with lung cancer risk in a Han Chinese population from southwestern China.

PloS one·2012
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Oct 5, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

Implementation of a fluorescence spatiotemporal modulation super-resolution microscope.

Luwei Wang, Jin Li, Yue Chen

    Optics Letters
    |February 1, 2022
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new fluorescence spatiotemporal modulation method for super-resolution microscopy (SRM). This technique achieves sub-100 nm resolution with low laser power, enabling cost-effective, multi-color live-cell imaging.

    More Related Videos

    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    10.0K
    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    10.9K

    Related Experiment Videos

    Last Updated: Oct 5, 2025

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.0K
    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    10.0K
    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    10.9K

    Area of Science:

    • Cellular Biology
    • Microscopy Techniques
    • Biophysics

    Background:

    • Super-resolution microscopy (SRM) is crucial for visualizing subcellular structures.
    • Live-cell imaging demands low illumination intensity and high resolution.
    • Existing SRM methods often require high laser power or complex setups.

    Purpose of the Study:

    • To introduce a novel, low-light super-resolution imaging method.
    • To enable long-term, non-invasive live-cell imaging.
    • To lay the groundwork for affordable multi-color SRM systems.

    Main Methods:

    • Developed a time-resolved detection technique: fluorescence spatiotemporal modulation.
    • Extracted highly resolved photons using a weighted difference between beam center and periphery photons.
    • Utilized a single laser source for the imaging process.

    Main Results:

    • Achieved sub-100 nanometer resolution.
    • Operated at low laser power (tens of microwatts).
    • Demonstrated a method requiring only one laser.

    Conclusions:

    • The fluorescence spatiotemporal modulation method offers high resolution with low light intensity.
    • This technique is suitable for non-invasive, long-term live-cell super-resolution imaging.
    • The single-laser requirement paves the way for more accessible multi-color SRM systems.