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Updated: Oct 4, 2025

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
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Hybridoma Screening by Antibody Capture: Detection of Intracellular Binding by Immunofluorescence

Edward A Greenfield

    Cold Spring Harbor Protocols
    |February 2, 2022
    PubMed

    Hybridoma screening by immunofluorescence stainings of whole cells can be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol, cells are attached to a solid support (glass slides), which makes them easy to manipulate and transfer between different reagent solutions.

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