Related Experiment Video
Updated: Oct 4, 2025
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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
Published on: May 10, 2024
937
CRISPR-Cas3-based diagnostics for SARS-CoV-2 and influenza virus
Kazuto Yoshimi1,2, Kohei Takeshita3, Seiya Yamayoshi4
1Division of Animal Genetics, Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
Iscience
|February 7, 2022
Summary
A new CRISPR-Cas3 diagnostic tool, CONAN, rapidly detects SARS-CoV-2 and identifies mutations in influenza viruses. This low-cost, instrument-free method enables quick point-of-care testing for infectious diseases.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR-based diagnostics (CRISPR-dx) utilize Cas12 and Cas13 enzymes for pathogen detection.
- Existing CRISPR systems are effective but newer applications are being explored.
Purpose of the Study:
- To develop a rapid, low-cost, instrument-free diagnostic method for SARS-CoV-2 using CRISPR-Cas3.
- To evaluate the potential of CRISPR-Cas3 for detecting specific mutations in influenza A virus (IAV) variants.
Main Methods:
- Utilized collateral single-stranded DNA cleavage activity of Class 1 type I CRISPR-Cas3.
- Developed a CRISPR-Cas3-mediated assay named Cas3-Operated Nucleic Acid detectioN (CONAN).
- Tested CONAN for detection of SARS-CoV-2 in clinical samples and IAV mutations.
Main Results:
- CONAN provides rapid (within 40 minutes) detection of SARS-CoV-2.
- The assay demonstrated specific detection of single-base-pair mutations in IAV variants.
- CONAN functions as a low-cost, instrument-free diagnostic tool.
Conclusions:
- CRISPR-Cas3 offers a promising platform for developing rapid point-of-care diagnostics.
- CONAN facilitates timely diagnosis of SARS-CoV-2 and identification of drug-resistant IAV strains.
- This technology can improve patient management in hospital settings.
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