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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

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Fluorescent probe strategy for live cell distinction.

Xiao Liu1,2, Young-Tae Chang1,2

  • 1Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673, Republic of Korea. ytchang@postech.ac.kr.

Chemical Society Reviews
|February 9, 2022
PubMed
Summary
This summary is machine-generated.

Fluorescent probes offer advanced live cell discrimination by targeting intracellular biomarkers, surpassing traditional antibody methods. This review details various fluorescent probe strategies for cell-type distinction and their future development.

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Area of Science:

  • Biotechnology and Biomedical Engineering
  • Cell Biology and Imaging
  • Chemical Biology

Background:

  • Live cell discrimination is crucial for understanding complex biological systems.
  • Conventional methods using antibodies rely on surface biomarkers, limiting intracellular analysis.
  • Fluorescent probes enable intracellular biomarker detection, expanding discrimination options.

Purpose of the Study:

  • To review and outline concepts and mechanisms of fluorescent probe strategies for live cell discrimination.
  • To introduce applications of these strategies in cell-type distinction.
  • To discuss the advantages and challenges of fluorescent probe-based methods.

Main Methods:

  • Categorization of fluorescent probe strategies based on target differences: protein-oriented (POLD), carbohydrate-oriented (COLD), DNA-oriented (DOLD), gating-oriented (GOLD), metabolism-oriented (MOLD), and lipid-oriented (LOLD).
  • Explanation of probe retention mechanisms: interaction with biomolecules, transporter accumulation, and metabolic participation.
  • Review of existing literature on applications and advancements in fluorescent probe technology for cell discrimination.

Main Results:

  • Fluorescent probes provide a versatile approach for live cell discrimination by utilizing intracellular targets.
  • Diverse strategies exist, targeting proteins, carbohydrates, DNA, cellular gating, metabolism, and lipids.
  • These methods offer unique chemical principles for enhanced specificity and broader application scope.

Conclusions:

  • Fluorescent probe strategies present a powerful alternative to antibody-based methods for live cell discrimination.
  • Further development of cell-type-specific probes is facilitated by understanding these diverse mechanisms.
  • This review offers a new perspective on fluorescent probe strategies, promoting innovation in cell biology research.