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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Updated: Oct 3, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

Recent Advances on DNAzyme-Based Sensing.

Zhimei Huang¹, Xiangnan Wang², Zhenkun Wu¹

¹State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, P. R. China.

Chemistry, an Asian Journal

|February 14, 2022

Summary

This summary is machine-generated.

RNA-cleaving DNAzymes offer sensitive and programmable biosensing. This review covers new DNAzyme discovery and applications for detecting biomolecules in vitro and in vivo.

Keywords:

DNAzyme bioimaging chemical modification in vitro selection sensing

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Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

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Published on: February 9, 2024

Detection of Bacteria Using Fluorogenic DNAzymes

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Published on: September 17, 2016

Area of Science:

Biochemistry
Molecular Biology
Nanotechnology

Background:

RNA-cleaving DNAzymes are highly sensitive and programmable tools.
In vitro selection allows flexible discovery of novel DNAzymes.
DNAzyme-based sensors are gaining traction for biomolecule detection.

Purpose of the Study:

To review recent advancements in DNAzyme-based sensing applications.
To discuss strategies for expanding the DNAzyme library.
To highlight applications in biomolecule detection and future perspectives.

Main Methods:

In vitro selection for discovering new DNAzymes.
Chemical modifications to enhance DNAzyme functionality.
Review of recent literature on DNAzyme sensor applications.

Main Results:

Two primary strategies for DNAzyme library expansion identified: in vitro selection and chemical modification.
DNAzyme-based sensors demonstrate effective detection of various biomolecules.
Applications span both in vitro and in vivo detection scenarios.

Conclusions:

DNAzyme-based sensors show significant promise due to their sensitivity and programmability.
Continued development in DNAzyme discovery and modification will expand their utility.
Future research should address challenges and explore new directions in DNAzyme sensing.