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Quantitative Elasticity of Flexible Polymer Chains Using Interferometer-Based AFM.

Vikhyaat Ahlawat1, Surya Pratap S Deopa1, Shivprasad Patil1

  • 1Department of Physics, Indian Institute of Science Education and Research (IISER) Pune, Pashan Road, Pune 411008, India.

Nanomaterials (Basel, Switzerland)
|February 15, 2022
PubMed
Summary
This summary is machine-generated.

We developed a new atomic force microscope (AFM) method using fiber interferometry to accurately measure polymer elasticity. This technique provides reliable polymer persistence length, validating models in good solvents but questioning their use in poor solvents.

Keywords:
AFMoscillatory responsepersistence length

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Area of Science:

  • Polymer Physics
  • Biophysics
  • Materials Science

Background:

  • Atomic Force Microscopy (AFM) is widely used to measure polymer elasticity.
  • Accurate AFM elasticity measurements are often limited by assumptions in cantilever dynamics.
  • Existing methods can yield anomalous results, particularly in different solvent conditions.

Purpose of the Study:

  • To develop a more accurate method for estimating single polymer chain elasticity using AFM.
  • To investigate the influence of solvent quality on polymer elasticity measurements.
  • To validate the worm-like chain (WLC) model under various conditions.

Main Methods:

  • Utilized a home-built fiber-interferometry-based detection system for cantilever oscillation measurement.
  • Employed AFM-based oscillatory experiments by oscillating the cantilever base.
  • Extracted stiffness-extension profiles for polymers in different solvents.

Main Results:

  • The new method provided physically acceptable persistence length values for polyethylene glycol (PEG) in good solvent, aligning with magnetic tweezers data.
  • Significant deviations were observed between the new method and conventional pulling experiments for PEG in good solvent and polystyrene (PS) in good solvent.
  • No deviation was found for PS in a poor solvent, suggesting solvent quality impacts measurement accuracy.

Conclusions:

  • The fiber-interferometry AFM method offers unambiguous and physically meaningful measurements of polymer persistence length.
  • The worm-like chain (WLC) model is validated for polymers in good solvents using this technique.
  • Caution is advised when applying the WLC model to polymers in poor solvents based on conventional pulling experiments.