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Updated: Sep 25, 2026

Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
Published on: April 1, 2014
[Lipid inclusions in cells and their changes during the desiccation and reactivation of yeast organisms]
Abstract:
The formation of lipid granules in Saccharomyces cerevisiae cells was shown to begin in the exponential phase of the culture growth and to be associated with the activity of the endoplasmic reticulum. Lipid granules can be formed (1) when the endoplasmic reticulum cisterns extend and (2) when the endoplasmic reticulum membranes separate relatively small regions of the cell cytoplasm. Yeast cells contain spherosomes which differ in their structure. Lipid granules merge together upon dehydration of yeast cells. The authors discuss possible participation of these granules in the reparation of damages of the intracellular membranes.
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