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Updated: Oct 3, 2025

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Published on: February 7, 2022

An optimized method for tissue glycogen quantification.

Kyle J Schaubroeck¹, Brooks P Leitner¹, Rachel J Perry¹

¹Departments of Cellular & Molecular Physiology and Internal Medicine (Endocrinology), Yale School of Medicine, New Haven, Connecticut, USA.

Physiological Reports

|February 18, 2022

Summary

This summary is machine-generated.

This study presents a new, cost-effective method for measuring tissue glycogen. The streamlined protocol accurately quantifies glycogen, even at low concentrations, aiding metabolic research.

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Related Experiment Videos

Last Updated: Oct 3, 2025

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Published on: February 7, 2022

Biochemical Titration of Glycogen In vitro

Biochemical Titration of Glycogen In vitro

Published on: November 24, 2013

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

Published on: February 8, 2022

Area of Science:

Biochemistry
Metabolic Research
Analytical Chemistry

Background:

Glycogen is crucial for glucose homeostasis and energy during exercise.
Accurate glycogen measurement is vital for metabolic research but current methods have limitations.
Existing methods suffer from poor sensitivity, high sample requirements, and technical variability.

Purpose of the Study:

To develop a streamlined and versatile protocol for glycogen extraction and quantification.
To overcome the limitations of previous glycogen detection methods.
To enable precise glycogen measurement, especially at low concentrations and in various physiological states.

Main Methods:

A streamlined glycogen extraction protocol involving a single centrifugation step.
An optimized phenol-sulfuric acid assay for glycogen quantification.
Validation of the method using small tissue sample masses (20 mg).

Main Results:

The method allows glycogen extraction from as little as 20 mg of tissue.
The assay demonstrates high precision (Intra-assay %CV 5-10%) and sensitivity.
The cost of materials is approximately 10 cents per sample.

Conclusions:

This optimized protocol offers a precise, sensitive, and cost-effective way to measure ex vivo tissue glycogen.
The method is suitable for assessing glycogen content across a wide range of concentrations, including extremes.
It provides a valuable tool for advancing metabolic research, particularly concerning hypoglycemia and exercise physiology.