Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

YOLO-Drop: A Deep Learning Model Enabling Accurate, High-Throughput Image Analysis for Droplet Digital Immunoassay at Attomolar Concentrations.

Analytical chemistry·2026

Same author

Enzyme-Activated Programmable Theranostic Platform for Spatiotemporal Imaging of Intracellular MicroRNA and On-Demand Manipulation-Mediated Therapy.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Biological Logic-Gated DNA Self-Assembly Strategy for Precision Tumor Imaging and Selective Activation of the cGAS-STING Pathway.

ACS applied materials & interfaces·2026

Same author

Bioinspired miRNA-Responsive Ca<sup>2+</sup> Nanoregulator with Dual Interference Pathways and Self-Amplifying Cascade for Tumor-Targeted Mitochondrial Dysfunction.

ACS nano·2025

Same author

Dual-Mode Biosensing Strategy Leveraging Single-Atom Fe Nanozyme for Cellular Glutathione Detection.

ACS applied materials & interfaces·2025

Same author

A glutathione-activated programmable DNA device for visual microRNA-210-enhanced photodynamic therapy of hypoxic lung cancer.

Chemical communications (Cambridge, England)·2025

Search research articles

Related Experiment Video

Updated: Oct 2, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Spatially Resolved, Error-Robust Multiplexed MicroRNA Profiling in Single Living Cells.

Wei Wei¹, Wenhao Dai¹, Fan Yang¹

¹Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, 100083, Beijing, China.

Angewandte Chemie (International Ed. in English)

|February 23, 2022

Summary

This study introduces FluoELs, a novel method for simultaneously imaging multiple microRNAs (miRNAs) in living cells. This breakthrough enables accurate quantification and spatial resolution of miRNAs, aiding disease mechanism research.

Keywords:

Error Correction Living Cell Imaging MicroRNA Multiplexed Detection Nanoparticles Spatial Resolution

More Related Videos

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Related Experiment Videos

Last Updated: Oct 2, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Simultaneous imaging of multiple microRNAs (miRNAs) in living cells is difficult due to limitations in spectrally distinct fluorophores and non-cytotoxic methods.
Existing techniques struggle with accurate quantification and spatial resolution of multiple miRNA targets within individual cells.

Purpose of the Study:

To develop a multiplexed, error-robust, and non-cytotoxic method for simultaneous miRNA imaging in living cells.
To enable accurate quantification and spatial resolution of multiple miRNAs for single-cell analysis.

Main Methods:

Development of fluorophores encoded error-corrected labels (FluoELs) using core-shell silica nanoparticles.
FluoELs utilize proportional dual fluorophores for encoding and a constant single fluorophore for error-corrected quantification.
Modification of nanoparticles with molecular beacon probes for miRNA detection.

Main Results:

FluoELs demonstrated low cytotoxicity in living cells.
Accurate quantification and spatial resolution of nine breast-cancer-related miRNAs were achieved.
The method allowed for the evaluation of miRNA coordination and expression profiles.

Conclusions:

FluoELs provide a robust platform for multiplexed miRNA imaging with error-correction capabilities.
This technology facilitates single-cell analysis of miRNA expression profiles and disease-associated molecular mechanisms.
FluoELs represent a significant advancement in cellular miRNA research and diagnostics.