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MiR-24-3p Conservatively Regulates Muscle Cell Proliferation and Apoptosis by Targeting Common Gene CAMK2B in Rat and

Ge Yang1, Mingli Wu1, Xinqi Liu1

  • 1Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China.

Animals : an Open Access Journal From MDPI
|February 25, 2022
PubMed

Insights

MicroRNAs (miRNAs) like miR-24-3p are conserved regulators of skeletal muscle development across species. This study confirms miR-24-3p promotes muscle cell proliferation and regulates key genes, including WNT4, CAMK2B, and TCF7.

Area of Science:

  • Molecular Biology
  • Genetics
  • Animal Science

Background:

  • Skeletal muscle development is crucial for meat animal growth.
  • MicroRNAs (miRNAs) are known regulators of cellular functions, including muscle development.
  • Limited information exists on conserved miRNAs regulating muscle development across species.

Purpose of the Study:

  • To identify conserved miRNAs involved in skeletal muscle development.
  • To investigate the role of miR-24-3p in regulating muscle cell proliferation and apoptosis.
  • To elucidate the target genes and pathways influenced by miR-24-3p in muscle development.

Main Methods:

  • Comparative miRNA transcriptome sequencing of muscle tissue from cattle, rats, goats, and pigs.
  • Cell proliferation and apoptosis assays (CCK-8) and gene expression analysis (RT-qPCR) in C2C12 and primary bovine muscle cells.
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis.
  • Dual-luciferase reporter assays to validate miRNA-target gene interactions.

Main Results:

  • miR-24-3p was identified as a potentially conserved regulator of muscle development across the studied species.
  • miR-24-3p positively regulates proliferation and apoptosis in both rat (mmu-miR-24-3p) and bovine (bta-miR-24-3p) muscle cells.
  • GO and KEGG analyses revealed enrichment of miR-24-3p targets in muscle development-related pathways.
  • WNT4, CAMK2B, and TCF7 were validated as direct targets of miR-24-3p, involved in the Wnt signaling pathway.

Conclusions:

  • miR-24-3p plays a conserved, positive regulatory role in skeletal muscle development.
  • The Wnt signaling pathway, mediated by targets like WNT4, CAMK2B, and TCF7, is a key mechanism through which miR-24-3p influences muscle growth.
  • These findings enhance understanding of miRNA-mediated muscle development regulation.
Keywords:
C2C12 cellCAMK2BCCK-8double luciferase testmiR-24-3pmuscle development

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