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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
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Correction: Chhalliyil et al. A Real-Time Quantitative PCR Method Specific for Detection and Quantification of the

Pradheep Chhalliyil1, Heini Ilves2, Sergei A Kazakov2

  • 1Health Research Institute, 505 Dimick Drive, P.O. Box 370, Fairfield, IA 52556, USA.

Foods (Basel, Switzerland)
|February 25, 2022
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...