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Microfluidic Model to Mimic Initial Event of Neovascularization
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Angiogenesis and Functional Vessel Formation Induced by Interstitial Flow and Vascular Endothelial Growth Factor

Yufang Liu1,2,3, Jiao Li1,2, Jiasheng Zhou3

  • 1Department of Oral Pathology, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Tianjin Road No. 2, Huangpu District, Shanghai 200001, China.

Micromachines
|February 25, 2022
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Summary

Vascular endothelial growth factor (VEGF) isoforms drive blood vessel growth. VEGF165 is most effective at promoting sprouting and functional vessel formation, as shown on a microfluidic chip.

Keywords:
VEGFangiogenesisinterstitial flowmicrofluidic

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Area of Science:

  • Cardiovascular Biology
  • Biomedical Engineering
  • Cellular and Molecular Medicine

Background:

  • Angiogenesis, the formation of new blood vessels, is crucial in both normal physiological and disease states.
  • Understanding the molecular mechanisms and factors influencing angiogenesis is vital for therapeutic development.

Purpose of the Study:

  • To utilize a microfluidic chip to investigate the process of angiogenesis.
  • To assess the roles of vascular endothelial growth factor (VEGF) and its isoforms in angiogenic sprouting and functional vessel formation.

Main Methods:

  • Development and application of a microfluidic chip to model angiogenesis.
  • Assessment of angiogenic sprouting, vessel formation, sprout length, number, perfusion, and connectivity.
  • Comparative analysis of different vascular endothelial growth factor (VEGF) isoforms (VEGF121, VEGF165, VEGF189).

Main Results:

  • Vascular endothelial growth factor (VEGF) initiated vascular sprouts, with interstitial flow directing their growth.
  • Different VEGF isoforms exhibited distinct angiogenic properties.
  • VEGF165 demonstrated the highest efficacy in inducing both vascular sprouting and functional vessel formation.

Conclusions:

  • The microfluidic chip serves as a valuable platform for studying angiogenesis.
  • VEGF165 is a key driver of functional blood vessel development.
  • This model can be used to evaluate angiogenic factors, drugs, and underlying mechanisms.