CoO Nanozymes with Multiple Catalytic Activities Regulate Atopic Dermatitis

Herein, we prepared CoO nanozymes with three types of enzyme catalytic activities for the first time, which have SOD-like, CAT-like, and POD-like catalytic activities. This is the first study to report the preparation of CoO nanoparticles with three types of enzyme catalytic activities by the one-pot method. By modifying the surface of CoO nanozymes with a carboxyl group, its biocompatibility enhanced, so it can be used in the field of life sciences. In vitro cytotoxicity and anti-H₂O₂-induced ROS experiments proved that CoO nanozymes can protect HaCaT cells against ROS and cytotoxicity induced by H₂O₂. In addition, an atopic dermatitis (AD) mouse model was established by topical application of MC903, which verified the anti-inflammatory effect of CoO nanozymes on the AD mouse model. Traditional drugs for the treatment of AD, such as dexamethasone, have significant side-effects. The side-effects include skin burns, telangiectasias, and even serious drug dependence. CoO nano-enzymes have a low cytotoxicity and its multiple enzyme-like catalytic activities can effectively protect cells and tissues in ROS environments, which proves that CoO nano-enzymes have high application potential in the field of anti-inflammation.