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Photoanode-supported cathodic immunosensor for sensitive and specific detection of human chorionic gonadotropin
Yibin Lu1, Hao Wang1, Xiao-Mei Shi2
1Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
Analytica Chimica Acta
|March 1, 2022
Summary
A novel photoelectrochemical (PEC) immunosensor was developed for sensitive detection of human chorionic gonadotropin (HCG). This enhanced cathodic immunosensor utilizes a novel photoanode for accurate biomarker detection in biological samples.
Area of Science:
- Electrochemistry
- Biosensing
- Materials Science
Background:
- Accurate biomarker detection is crucial for immunoassays.
- Existing methods for detecting biomarkers like human chorionic gonadotropin (HCG) require enhancement in sensitivity and specificity.
- Photoelectrochemical (PEC) sensing offers a promising avenue for sensitive biological detection.
Purpose of the Study:
- To design and fabricate a novel photoanode-supported cathodic immunosensor.
- To achieve sensitive and specific detection of human chorionic gonadotropin (HCG).
- To explore the potential of enhanced PEC immunosensors for biological sample analysis.
Main Methods:
- Fabrication of a TiO2 nanotube with N doping (TiO2:N) and AgInS2 quantum dots (QDs) photoanode.
- Preparation of a carbon nanotubes (CNTs) decorated with Pt nanoparticles (NPs) sensing cathode modified with HCG antibody (Ab).
- Utilizing a photoelectrochemical (PEC) system where the photoanode converts light signals and the cathode detects immune recognition events via steric hindrance.
Main Results:
- The developed immunosensor demonstrated high sensitivity and specificity for HCG detection.
- The PEC system exhibited excellent photoelectric properties and good anti-interference ability.
- The steric hindrance effect at the cathode significantly altered the current signal upon HCG binding.
Conclusions:
- The novel photoanode-supported cathodic immunosensor enables sensitive and specific HCG detection.
- This strategy offers a potential pathway for developing other enhanced PEC immunosensors for biological applications.
- The developed sensor shows promise for accurate biomarker detection in complex biological samples.
