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Updated: Oct 1, 2025

Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering

Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering

Published on: November 25, 2011

Self-Assembling Toroidal Cell Constructs for Tissue Engineering Applications.

Austin Worden¹, Mark J Uline^2,3, Tarek Shazly^2,4

¹Department of Cell Biology and Anatomy, University of South Carolina, School of Medicine, Columbia, SC29209, USA.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

|March 2, 2022

Summary

This summary is machine-generated.

This study reveals that cellularized collagen hydrogels rapidly form self-assembled cell toroids within hours, offering a novel model for studying cell migration and tissue development.

Keywords:

collagen hydrogels self-organizing stem cells tissue engineering

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Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

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Last Updated: Oct 1, 2025

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Published on: April 2, 2018

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

Area of Science:

Biomaterials Science
Cell Biology
Tissue Engineering

Background:

Developing tissues rely on complex 3D arrangements of cells and extracellular matrix (ECM).
Migrating cells encounter diverse signals (chemokines, ECM, growth factors, physical forces) that are challenging to study collectively.
Existing models often examine these signals in isolation, limiting our understanding of cellular integration.

Purpose of the Study:

To investigate the rapid formation and characteristics of self-organizing cell toroids in a tunable collagen hydrogel model.
To explore how different collagen subtypes and hydrogel compositions influence toroid formation and tissue remodeling.
To compare the remodeling behavior of toroids with cells embedded throughout the hydrogel.

Main Methods:

Development of a self-organizing cellularized collagen hydrogel model.
Time-lapse imaging to observe toroid formation kinetics (as early as 3 hours).
Rheology and scanning electron microscopy (SEM) to analyze hydrogel remodeling and structure.
Assessment of toroid formation on various collagen subtypes and hydrogel compositions.
Investigation of heterogeneous toroid formation with mixed cell populations.

Main Results:

Cellularized collagen hydrogels rapidly form self-assembled cell toroids within 3 hours.
Toroid formation is influenced by collagen subtype and hydrogel composition.
Toroid gels exhibit distinct remodeling patterns compared to uniformly cellularized gels, forming crater-like structures.
Heterogeneous toroids with cell clusters form when multiple cell types are mixed prior to seeding.

Conclusions:

The developed hydrogel model enables rapid, self-organized formation of cell toroids, mimicking in vivo cellular environments.
This model provides a platform for studying how cells integrate multiple environmental cues during tissue development and migration.
The findings highlight differences in tissue remodeling between organized cellular structures (toroids) and uniformly distributed cells.