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Multi-resistive pulse sensor microfluidic device.

Marcus Pollard1, Rushabh Maugi1, Mark Platt1

  • 1School of Science, Loughborough University, Epinal Way, LE11 3TU, UK. m.platt@lboro.ac.uk.

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Summary
This summary is machine-generated.

This study introduces an advanced, tuneable flow resistive pulse sensor for simultaneous particle size measurement. The novel multi-nanopore design prevents blockages and measures particles from 0.1 to 30 μm.

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Area of Science:

  • * Nanotechnology and Microfluidics
  • * Particle Characterization

Background:

  • * Resistive pulse sensors are vital for characterizing particles but often face limitations in size range and susceptibility to blockages.
  • * Current methods require sample preparation or filtration to match sensor dimensions, hindering complex sample analysis.
  • * Integration into microfluidic devices simplifies handling and increases throughput but doesn't fully address size limitations.

Purpose of the Study:

  • * To develop an advanced, tuneable flow resistive pulse sensor with enhanced versatility and blockage resistance.
  • * To create a multi-nanopore sensor capable of simultaneously measuring a broad range of particle sizes.
  • * To demonstrate a novel sensor configuration for efficient particle characterization in complex matrices.

Main Methods:

  • * Utilization of additively manufactured components for a tuneable resistive pulse sensor.
  • * Integration of existing nanopore fabrication techniques (e.g., glass pipettes) into a single device.
  • * Design of a multi-pore sensor with controlled fluid flow and sensors arranged in series.

Main Results:

  • * Development of a multi-nanopore sensor capable of measuring particles from 0.1 to 30 μm simultaneously.
  • * Successful demonstration of a dual-pore system combining a tuneable sensor (Sensor 1, ≥10 μm) and a fixed nanopore sensor (Sensor 2, ≥100 nm).
  • * Simultaneous measurement of 1 μm and 10 μm particles without sensor blockage.

Conclusions:

  • * The novel multi-nanopore resistive pulse sensor overcomes limitations of traditional sensors, enabling broader particle size analysis.
  • * Additive manufacturing and modular design facilitate easy component replacement, cleaning, and integration of diverse nanopore technologies.
  • * This versatile platform offers a robust solution for complex sample analysis, reducing the need for extensive sample preparation.