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Updated: Aug 6, 2026

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Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids
Published on: September 25, 2016
Summary
This study proposes preliminary somatic nutrient requirements for adult animals, focusing on energy and amino acids. Further research is needed to establish precise requirements for specific production functions and species.
Area of Science:
- Animal Nutrition
- Comparative Physiology
- Nutrient Metabolism
Background:
- Existing data on somatic nutrient requirements are limited.
- Interspecies generalizations are needed for research focus.
- Maintenance requirements are calculated per kilogram of metabolic body size.
Purpose of the Study:
- To propose tentative preliminary figures for somatic nutrient requirements.
- To provide a focus for future research on nutrient requirements.
- To highlight data gaps in nutrient requirements.
Main Methods:
- Calculated energy requirements by accounting for metabolizable energy, heat losses, and VFA metabolism.
- Utilized intravenous administration data for amino acid values.
- Excluded water-soluble vitamins due to expected similarity to whole-body requirements, except for thiamin.
Main Results:
- Presented interspecies generalizations for somatic nutrient requirements.
- Included nutrients with available experimental data.
- Identified a need for definitive amino acid and water-soluble vitamin data.
Conclusions:
- Preliminary somatic nutrient requirements are proposed.
- Definitive data for amino acid requirements in productive functions are crucial.
- Further research on water-soluble vitamin requirements is necessary to avoid arbitrary supplementation.
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