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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

417
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
417
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

574
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
574
Electrophoresis: Overview01:20

Electrophoresis: Overview

2.4K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Updated: Oct 1, 2025

Picoinjection of Microfluidic Drops Without Metal Electrodes
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3-D printed injection system for capillary electrophoresis.

Bonnie Jaskowski Huge1, Kevin Young1, Caitlin Kerr1

  • 1Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA. bjaskows@nd.edu.

Analytical Methods : Advancing Methods and Applications
|March 7, 2022
PubMed
Summary

We developed a compact, 3D-printed capillary electrophoresis injector for single-sample analysis, ideal for method development and training. This affordable system offers precise pressure or electrokinetic injection with excellent detection limits.

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Area of Science:

  • Analytical Chemistry
  • Instrumentation Science

Background:

  • Commercial capillary electrophoresis (CE) systems are often large, complex, and expensive.
  • There is a need for simpler, more accessible CE systems for specific applications like method development and education.

Purpose of the Study:

  • To design and evaluate a compact, manually operated capillary electrophoresis injector.
  • To provide an affordable and user-friendly alternative to commercial CE systems for single-sample analysis.

Main Methods:

  • A two-part injector was fabricated using 3D printing (STL and STEP files provided).
  • Conventional machining was used for precise component fitting (electrode, capillary, gas line).
  • The system was coupled with a laser-induced fluorescence detector for capillary zone electrophoresis experiments.

Main Results:

  • The system supported both pressure and electrokinetic sample injection.
  • Electrokinetic injection achieved a 5-order dynamic range, 5 pM concentration LOD, and 1 zmol mass LOD.
  • Pressure injection demonstrated a 4-order dynamic range, 2 pM concentration LOD, and 10 zmol mass LOD.

Conclusions:

  • The 3D-printed CE injector is a cost-effective and versatile tool for method development and training.
  • The system offers high sensitivity and a wide dynamic range, comparable to more complex commercial instruments.