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Decellularized Pig Kidney with a Micro-Nano Secondary Structure Contributes to Tumor Progression in 3D Tumor Model
Shuangjia Yang1, Le Zheng1, Zilong Chen1
1State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
Materials (Basel, Switzerland)
|March 10, 2022
Summary
This study developed a 3D scaffold from decellularized pig kidney extracellular matrix (dECM). This biocompatible scaffold supports cancer cell adhesion and proliferation, offering a promising 3D tumor model.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cancer Research
Background:
- Cancer remains a leading cause of mortality due to tumor microenvironment complexity.
- Conventional 2D tumor models inadequately replicate native tumor tissues.
- Extracellular matrix (ECM) nanofibers are crucial components of the cellular microenvironment.
Purpose of the Study:
- To develop a biocompatible 3D scaffold using decellularized pig kidney extracellular matrix (dECM).
- To enhance the mechanical and physical properties of the dECM scaffold via chemical crosslinking.
- To evaluate the scaffold's potential for constructing a 3D human breast cancer model.
Main Methods:
- Pig kidneys were processed to obtain decellularized extracellular matrix (dECM) scaffolds.
- Chemical crosslinking was employed to improve scaffold mechanical properties.
- MCF-7 human breast cancer cells were cultured on the scaffold to assess biocompatibility and tumor modeling potential.
Main Results:
- The dECM scaffold exhibited nanostructures essential for cell functions.
- The scaffold demonstrated significant biocompatibility, promoting cell adhesion and proliferation.
- The nanostructured scaffold proved effective for constructing a 3D tumor model.
Conclusions:
- Decellularized pig kidney matrix scaffolds provide enhanced cell attachment sites.
- These scaffolds are conducive to cell adhesion, proliferation, and physiological activities.
- The developed scaffold is a promising platform for advanced 3D tumor model construction.
