Introducing ATR-FTIR Spectroscopy through Analysis of Acetaminophen Drugs: Practical Lessons for Interdisciplinary...

Area of Science:

• Analytical Chemistry
• Spectroscopy
• Chemical Education

Background:

• Infrared (IR) spectroscopy is crucial for chemical identification across scientific, pharmaceutical, and forensic fields.
• Undergraduate curricula often lack comprehensive training in optimizing IR spectroscopy parameters and data analysis for substance identification.
• Proficiency in correlating spectral bands to molecular vibrations and applying chemometrics is essential for quality control and counterfeit detection.

Purpose of the Study:

• To present an experimental laboratory practice for introductory teaching of IR spectroscopy.
• To train students in optimizing instrumental conditions for obtaining high-quality IR spectra.
• To enhance students' ability in substance identification using visual comparison and statistical analysis of IR spectra.

Main Methods:

• Development of a hands-on laboratory practice focused on IR spectroscopy.
• Optimization of experimental parameters for IR spectral acquisition.
• Application of chemometrics for spectral comparison, visualization, and classification.
• Analysis of commercial drugs, primarily acetaminophen, in solid and solution states.

Main Results:

• Students gained proficiency in optimizing IR instrumental conditions.
• Effective correlation of IR spectral bands with molecular vibrations was demonstrated.
• Successful application of chemometrics for comparing, visualizing, and classifying IR spectra was achieved.
• Students were able to identify substances based on visual spectra comparison and statistical analysis.

Conclusions:

• The developed laboratory practice effectively trains undergraduate students in IR spectroscopy for substance identification.
• The curriculum enhances students' skills in chemical elucidation and quality control applications.
• This training is valuable for students in chemistry, pharmacy, and forensic science degrees.