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Related Experiment Video

Updated: Sep 30, 2025

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

Repeated imaging through a multimode optical fiber using adaptive optics.

Carla C Schmidt^1,2, Raphaël Turcotte^3,2, Martin J Booth⁴

¹Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, United Kingdom.

Biomedical Optics Express

|March 14, 2022

Summary

This summary is machine-generated.

This study presents a novel method for high-quality fluorescence imaging in deep brain regions using multimode optical fibers (MMF). A custom headplate and adaptive optics enable precise MMF repositioning for repeated, reliable imaging.

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Area of Science:

Neuroscience
Optical Engineering
Biomedical Imaging

Background:

Multimode optical fibers (MMF) offer potential for minimally invasive deep brain fluorescence imaging.
Repeated imaging is crucial for studying synaptic roles in neurological function and disease.
Current MMF imaging requires calibration, which is invalidated by manual repositioning for repeated measurements.

Purpose of the Study:

To develop a robust method for high-quality fluorescence imaging using MMF that overcomes calibration issues associated with repeated repositioning.
To enable long-term, repeated imaging in the same brain region for neuroscience research.

Main Methods:

A two-step solution was implemented: a custom headplate for precise MMF implant reinsertion and sensorless adaptive optics to correct positional shifts.
The headplate ensures low-quality focusing upon reinsertion.
Adaptive optics corrects translational shifts to achieve high-quality imaging foci.

Main Results:

The combined approach successfully enabled fluorescence imaging after repeated removal and reinsertion of the MMF.
High-quality imaging foci were generated despite manual repositioning challenges.
The method addresses the calibration invalidation problem for longitudinal MMF imaging.

Conclusions:

This technique provides a reliable solution for repeated, high-quality fluorescence imaging of deep brain regions using MMF.
It facilitates long-term studies of brain function and disease by enabling consistent MMF placement.
The developed system enhances the utility of MMF for advanced neuroscience research.