Related Experiment Video
Updated: Sep 30, 2025
Capturing Common Fragile Site Breaks by Native γH2A.X ChIP
Published on: January 24, 2025
Characterization of DNA-PK-Bound End Fragments Using GLASS-ChIP
Rajashree A Deshpande1, Tanya T Paull2
1The Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
Abstract:
Endonucleolytic cleavage of DNA ends by the human Mre11-Rad50-Nbs1 (MRN) complex occurs in a manner that is promoted by DNA-dependent protein kinase (DNA-PK). A method is described to isolate DNA-PK-bound fragments released from chromatin in human cells using a modified Gentle Lysis and Size Selection chromatin immunoprecipitation (GLASS-ChIP) protocol. This method, combined with real-time PCR or next-generation sequencing, can identify sites of MRN endonucleolytic cutting adjacent to DNA-PK binding sites in human cells.
More Related Videos
05:58Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
Published on: August 20, 2018
12:24DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014