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Updated: Sep 29, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

DNA-based enzymatic systems and their applications.

Yunfei Jiao^1,2, Yingxu Shang¹, Na Li^1,2

¹CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, Beijing 100190, China.

|March 22, 2022

Summary

DNAzymes (Dzs) and DNA nanotechnology are revolutionizing biocatalysis. This review covers DNA-based enzymes, their catalytic activities, and applications in biosensing and nanomedicine.

Keywords:

Biocatalysis Biochemistry Biomolecules Enzymology Nanotechnology

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Area of Science:

Biochemistry
Nanotechnology
Synthetic Biology

Background:

DNA strands with unique secondary structures can act as catalysts (DNAzymes) and mimic enzymes.
DNA nanotechnology offers a platform for organizing and regulating these DNA-based enzymatic systems.

Purpose of the Study:

To review recent advancements in DNA-based enzymatic systems.
To highlight the development and applications of DNAzymes and DNA nanostructures in catalysis, biosensing, and biomedicine.

Main Methods:

Review of literature on DNAzymes and DNA nanotechnology.
Elaboration on G-quadruplex/hemin DNAzymes and their biosensor applications.
Depiction of DNA-based enzymatic cascade systems using DNA nanotechnology.

Main Results:

DNAzymes exhibit versatile catalytic activities, including oxidase and peroxidase mimicry.
DNA nanostructures enable the fabrication of efficient enzymatic cascade systems.
Catalytic DNA nanostructures show promise in biosensing and biomedical applications.

Conclusions:

DNA-based enzymatic systems are rapidly advancing, offering powerful tools for various applications.
Further research into DNAzymes and DNA nanotechnology holds significant potential for future innovations in biocatalysis and medicine.