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Gene Therapy

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Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...

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Related Experiment Video

Updated: Jan 10, 2026

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Genetically Encoded DNA Origami for CAR-T-Based Immunotherapy.

Jing Fan^1,2, Huarui Liu^1,2, Changping Yang^1,2

¹Henan Institute of Advanced Technology, School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, China.

|November 20, 2025

Summary

This summary is machine-generated.

Researchers developed genetically encoded DNA origami for CAR-T immunotherapy. This novel system delivers chimeric antigen receptor (CAR) genes into T cells, enabling tumor cell targeting and enhancing CAR-T therapy effectiveness.

Keywords:

CAR-T DNA nanotechnology drug delivery self-assembly tumor immunotherapy

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Generation of Human Chimeric Antigen Receptor Regulatory T Cells

Generation of Human Chimeric Antigen Receptor Regulatory T Cells

Published on: January 3, 2025

Area of Science:

Biotechnology
Immunotherapy
Nanotechnology

Background:

DNA origami is utilized in various biomedical fields like bioimaging and drug delivery.
The gene-encoding potential of DNA scaffolds in DNA origami remains largely unexplored.

Purpose of the Study:

To develop a universal strategy for creating genetically encoded DNA origami.
To apply this technology for CAR-T-based immunotherapy.

Main Methods:

Incorporating sense and antisense strands encoding the chimeric antigen receptor (CAR) into DNA origami scaffolds.
Utilizing the genetically encoded DNA origami as a template for lipid growth.
Decorating the lipid-coated DNA origami with CD3 antibodies for T cell targeting.

Main Results:

Genetically encoded DNA origami successfully functions as a template for lipid growth.
Lipid-coated DNA origami with CD3 antibody decoration efficiently targets and penetrates T cells.
Achieved successful CAR expression within T cells for tumor cell capture.

Conclusions:

A novel DNA origami-based delivery system for CAR-T therapy has been rationally designed.
This approach provides a new strategy for developing effective CAR-T therapies.
The genetically encoded DNA origami system enhances CAR-T immunotherapy potential.