Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reconstruction of Molecular Interaction Patterns from Endolysosomes in Ceramide-Depleted Cells.

Nano letters·2026

Same author

Endolysosomal Impact of Elevated Ceramide Levels Revealed by Optical and Ultrastructural Nanoprobing.

ACS nano·2026

Same author

Nano-Infrared Imaging and Spectroscopy of Animal Cells in Liquid Environment.

Small (Weinheim an der Bergstrasse, Germany)·2025

Same author

Surface-enhanced Raman spectroscopy: a half-century historical perspective.

Chemical Society reviews·2024

Same author

Surface Enhanced Nonlinear Raman Processes for Advanced Vibrational Probing.

ACS nano·2024

Same author

SERS microscopy as a tool for comprehensive biochemical characterization in complex samples.

Chemical Society reviews·2024

Search research articles

Related Experiment Video

Updated: Sep 29, 2025

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

Surface enhanced Raman scattering for probing cellular biochemistry.

Cecilia Spedalieri¹, Janina Kneipp¹

¹Humboldt-Universität zu Berlin, Department of Chemistry, Brook-Taylor-Str. 2, 12489 Berlin, Germany. janina.kneipp@chemie.hu-berlin.de.

|March 22, 2022

Summary

This summary is machine-generated.

Surface enhanced Raman scattering (SERS) allows sensitive and selective probing of biomolecules within living cells. This technique has advanced from basic observation to analyzing complex cellular interactions and processes.

More Related Videos

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Related Experiment Videos

Last Updated: Sep 29, 2025

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Area of Science:

Biophysics
Chemical Biology
Molecular Spectroscopy

Background:

Surface-enhanced Raman scattering (SERS) offers sensitive and selective detection of biomolecules.
Probing biochemical composition in living cells is crucial for understanding cellular functions.
Previous SERS applications focused on proof-of-principle observations of cellular states.

Purpose of the Study:

To review the advancements in SERS probing within living cells.
To highlight the evolution from basic biochemical status observation to complex interaction analysis.
To discuss the integration of SERS with other techniques for comprehensive cellular analysis.

Main Methods:

Live cell SERS combined with organelle targeting.
Spectroscopy of relevant molecular models.
Optimization of plasmonic nanostructures.
Application of machine learning for data analysis.

Main Results:

SERS has evolved to characterize molecule-nanostructure and molecule-molecule interactions.
Cellular processes involving diverse biomolecules and compartments can be studied.
Integration of SERS with advanced methods provides a unified view of cellular systems.

Conclusions:

SERS is a powerful bioanalytical tool for understanding cellular physiology.
Combining SERS with various approaches enhances its application in complex biological systems.
Future directions involve unifying information from individual biomolecules and the cell as a whole.