Influence of Humoral Factors on Hemostatic Properties of Blood In Vitro in Rats

An important role in the implementation of many physiological and pathological processes is played by the functional state of the components of the hemostasis system, the activity of which is largely determined by the influence of various humoral factors. We studied the effects of lactate, nitric oxide, and sodium glutamate on aggregation activity of platelets and coagulation properties of rat plasma in vitro. The most pronounced effect was produced by lactic acid, which manifested in a dose-dependent decrease in the parameters of ADP-induced platelet aggregation and a two-phase change in blood coagulability with progressive acidification of the medium. Nitric oxide induced less pronounced decrease in the aggregation activity of platelets. Sodium glutamate, on the contrary, stimulated platelet aggregation and similar to nitric oxide did not affect coagulation status of the plasma.