Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 28, 2025

Mechanical Stimulation of Chondrocyte-agarose Hydrogels
12:45

Mechanical Stimulation of Chondrocyte-agarose Hydrogels

Published on: October 27, 2012

11.8K

Emulating the chondrocyte microenvironment using multi-directional mechanical stimulation in a cartilage-on-chip.

Carlo Alberto Paggi1,2, Jan Hendriks1, Marcel Karperien1

  • 1Department of Developmental BioEngineering, TechMed Centre, and Organ-on-chip Centre, University of Twente, Enschede, The Netherlands. marcel.karperien@utwente.nl.

Lab on a Chip
|March 30, 2022
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocol to study the contribution of biomechanics in osteoarthritis development using a cartilage-on-chip model.

STAR protocols·2026
Same author

A Synovium-on-Chip Platform to Study Multicellular Interactions in Arthritis.

Advanced healthcare materials·2026
Same author

A Quantitative Printability Framework for Programmable Assembly of Pre-Vascular Patterns via Laser-Induced Forward Transfer.

Advanced healthcare materials·2025
Same author

Organ-on-chip technologies: Novel tools with the potential to revolutionize osteoarthritis research and clinical development.

Osteoarthritis and cartilage open·2025
Same author

Hydrogel-Based Bioinks for Coaxial and Triaxial Bioprinting: A Review of Material Properties, Printing Techniques, and Applications.

Polymers·2025
Same author

3D Single-Molecule Super-Resolution Imaging of Microfabricated Multiscale Fractal Substrates for Calibration and Cell Imaging.

ACS applied materials & interfaces·2025

Mechanical stimulation influences chondrocyte behavior, promoting extracellular matrix production. Multi-directional forces, specifically, enhance cartilage matrix deposition and gene expression for hyaline cartilage markers in vitro.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Mechanobiology

Background:

  • Articular cartilage chondrocytes respond to mechanical stimuli via pericellular matrix.
  • Understanding chondrocyte response to mechanical cues is crucial for cartilage repair and regeneration.

Purpose of the Study:

  • To investigate the impact of different mechanical stimulation types on chondrocyte phenotype and extracellular matrix (ECM) production.
  • To utilize a cartilage-on-chip system for controlled mechanical stimulation of chondrocytes.

Main Methods:

  • A cartilage-on-chip system was developed to apply compressive and multi-directional mechanical stimulation to 3D chondrocyte-laden agarose hydrogels.
  • Chondrocyte gene expression (COL2A1, COL1A1), inflammatory cytokine production (IL-6, IL-1β, TNF-α), and glycosaminoglycan (GAG) production were analyzed.

More Related Videos

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
07:23

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

Published on: September 13, 2019

6.7K
Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
08:04

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

Published on: April 25, 2013

14.6K

Related Experiment Videos

Last Updated: Sep 28, 2025

Mechanical Stimulation of Chondrocyte-agarose Hydrogels
12:45

Mechanical Stimulation of Chondrocyte-agarose Hydrogels

Published on: October 27, 2012

11.8K
A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
07:23

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

Published on: September 13, 2019

6.7K
Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
08:04

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

Published on: April 25, 2013

14.6K

Main Results:

  • Mechanical stimulation initially increased IL-6 production but minimally affected IL-1β and TNF-α.
  • Enhanced expression of COL2A1 (hyaline cartilage marker) and decreased COL1A1 (fibrotic marker) were observed, with multi-directional stimulation showing a greater effect.
  • Significant increase in GAG production and deposition of pericellular and interstitial matrices, mimicking in vivo conditions, were noted, particularly with multi-directional stimulation.

Conclusions:

  • Mechanical cues are critical for emulating the in vitro chondrocyte microenvironment.
  • Multi-directional mechanical stimulation is more effective in promoting hyaline cartilage matrix production compared to other tested stimuli.
  • The cartilage-on-chip system provides a valuable platform for studying chondrocyte mechanobiology and developing cartilage regeneration strategies.