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Updated: Feb 14, 2026

Destabilization of the Medial Meniscus and Cartilage Scratch Murine Model of Accelerated Osteoarthritis
Published on: July 6, 2022
Protocol to study the contribution of biomechanics in osteoarthritis development using a cartilage-on-chip model
Elodie Faure1, Veronique Chobaz1, Diego de Haro2
1Division of Rheumatology, Department of Musculoskeletal Medicine, Lausanne University Hospital, 1010 Lausanne, Switzerland.
Abstract:
Here, we present a protocol to study biomechanics and calcification in osteoarthritis using a cartilage-on-chip model. We describe steps for culturing human chondrocytes in 3D hydrogels and subjecting them to physiological loading. We then detail procedures for assessing spontaneous calcification via alizarin red S staining, gene expression using qPCR, protein expression by immunofluorescence, and protein secretome analysis through the Olink proximity extension assay. This protocol is adaptable and allows exploration of cartilage degeneration mechanisms under controlled conditions.
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