Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular evidence reveals unsustainable harvest of wild orchids.

Current biology : CB·2026
Same author

First chromosome-level genome assembly of the colonial chordate model Botryllus schlosseri (Tunicata).

GigaScience·2025
Same author

Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis: insights into tissue specialization and blastogenesis.

Development (Cambridge, England)·2025
Same author

Photoluminescence and Thermoluminescence Studies of Beta-Irradiated Ba<sub>3</sub>CdSi<sub>2</sub>O<sub>8</sub>:Tb<sup>3+</sup> Phosphor for LED and Dosimetry Applications.

Luminescence : the journal of biological and chemical luminescence·2025
Same author

A New Promising Silicate-Based Phosphor for Red Light and White Light Emitting Devices.

Journal of fluorescence·2025
Same author

Novel smart materials with high curie temperatures: Eu<sub>1.90</sub>Dy<sub>0.10</sub>Ge<sub>2</sub>O<sub>7</sub>, Eu<sub>1.90</sub>La<sub>0.10</sub>Ge<sub>2</sub>O<sub>7</sub> and Eu<sub>1.90</sub>Ho<sub>0.10</sub>Ge<sub>2</sub>O<sub>7</sub>.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2025

Related Experiment Video

Updated: Sep 28, 2025

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians
06:49

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians

Published on: March 14, 2014

11.7K

Studying Tunicata WBR Using Botrylloides anceps.

Arzu Karahan1, Esra Öztürk2, Berivan Temiz2

  • 1Institute of Marine Sciences, Middle East Technical University, Erdemli, Mersin, Turkey. arzukarahan@ims.metu.edu.tr.

Methods in Molecular Biology (Clifton, N.J.)
|April 1, 2022
PubMed
Summary

Colonial tunicates, like Botrylloides anceps, uniquely regenerate entire bodies from vascular fragments. This study details methods for collecting, breeding, and studying this remarkable whole-body regeneration (WBR) process in tunicates.

Keywords:
Botrylloides ancepsChordateDNA barcodingHistological sectionVascular buddingWhole-body regeneration

More Related Videos

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha
08:09

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Published on: August 6, 2019

9.2K
Cultivation of the Marine Pelagic Tunicate Dolioletta gegenbauri Uljanin 1884 for Experimental Studies
09:39

Cultivation of the Marine Pelagic Tunicate Dolioletta gegenbauri Uljanin 1884 for Experimental Studies

Published on: August 9, 2019

10.2K

Related Experiment Videos

Last Updated: Sep 28, 2025

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians
06:49

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians

Published on: March 14, 2014

11.7K
Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha
08:09

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Published on: August 6, 2019

9.2K
Cultivation of the Marine Pelagic Tunicate Dolioletta gegenbauri Uljanin 1884 for Experimental Studies
09:39

Cultivation of the Marine Pelagic Tunicate Dolioletta gegenbauri Uljanin 1884 for Experimental Studies

Published on: August 9, 2019

10.2K

Area of Science:

  • Marine biology
  • Invertebrate zoology
  • Developmental biology

Background:

  • Tunicates are marine invertebrates and the closest living relatives to vertebrates.
  • Colonial tunicates exhibit unique Whole-Body Regeneration (WBR) via vascular budding, a trait not found in other chordates.
  • Botrylloides anceps can regenerate a complete adult from a vascular fragment within two weeks.

Purpose of the Study:

  • To provide detailed protocols for the study of Whole-Body Regeneration (WBR) in Botrylloides anceps.
  • To establish standardized methods for collecting, identifying, and breeding Botrylloides anceps in a laboratory setting.
  • To outline techniques for monitoring WBR and performing histological analyses on regenerated tissues.

Main Methods:

  • Collection and species confirmation of Botrylloides anceps colonies.
  • Laboratory breeding and maintenance of tunicate colonies.
  • Monitoring of Whole-Body Regeneration (WBR) post-amputation.
  • Histological staining of cryosections for tissue analysis.

Main Results:

  • Established protocols for the successful collection and laboratory breeding of Botrylloides anceps.
  • Demonstrated methods for observing and quantifying Whole-Body Regeneration (WBR) in this species.
  • Validated histological techniques for examining regenerated tissues.

Conclusions:

  • Botrylloides anceps serves as a valuable model organism for studying Whole-Body Regeneration (WBR) in chordates.
  • The presented protocols facilitate reproducible research into the mechanisms of tunicate regeneration.
  • Further research using these methods can elucidate the genetic and cellular basis of WBR.