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Updated: Sep 28, 2025

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Published on: September 13, 2013
Molecular probes for cellular imaging of post-translational proteoforms
Surased Suraritdechachai1, Benya Lakkanasirorat1, Chayasith Uttamapinant1
1School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC) Rayong Thailand chayasith.u@vistec.ac.th.
Visualizing protein post-translational modifications (PTMs) in living cells is challenging. This review covers new molecular imaging tools for detecting PTMs and understanding their roles in cell signaling and protein function.
Area of Science:
- Cellular biology
- Molecular imaging
- Biochemistry
Background:
- Protein post-translational modifications (PTMs) critically regulate protein function and cellular processes.
- Understanding PTM dynamics is essential for deciphering signaling pathways.
- Visualizing PTMs in a site-specific manner within living cells remains a significant challenge.
Purpose of the Study:
- To review recent advancements in molecular imaging tools for detecting diverse post-translational proteoforms.
- To highlight the applications of these tools in studying the precise roles of PTMs in cellular protein function.
Main Methods:
- Review of emerging molecular imaging technologies.
- Analysis of techniques for site-specific PTM detection.
- Examination of live-cell imaging applications for proteoforms.
Main Results:
- Development of novel molecular probes and imaging strategies for PTM detection.
- Demonstration of visualizing PTMs in individual cells with high specificity.
- Successful application of imaging tools to elucidate PTM roles in biological regulation.
Conclusions:
- Advanced molecular imaging tools offer unprecedented capabilities for studying PTMs in living cells.
- These tools are crucial for dissecting the functional impact of specific PTM states on protein activity.
- Future developments in imaging technologies will further enhance our understanding of PTM-mediated cellular regulation.
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